12. What is insertional inactivation?
Dear Student,
Please find below the solution to the asked query:
Insertional inactivation is a technique employed in recombinant DNA technology. It refers to the inactivation of a gene by inserting a DNA fragment into the middle of the coding sequence. Thus, the products from the inactivated gene will not work as the sequence gets disrupted. For example, in case of pBR322, which has genes that confer resistance to ampicillin and tetracyclin antibiotics. When a genetic region is disrupted by integration into the antibiotic resistance conferring genetic region say the tetracyclin of pBR322, the gene function is lost.This in turn helps in the selection of recombinant colonies as the recombinant colonies would now only grow in ampicillin containing medium because now it is sensitive to tetracyclin.
Regards
Please find below the solution to the asked query:
Insertional inactivation is a technique employed in recombinant DNA technology. It refers to the inactivation of a gene by inserting a DNA fragment into the middle of the coding sequence. Thus, the products from the inactivated gene will not work as the sequence gets disrupted. For example, in case of pBR322, which has genes that confer resistance to ampicillin and tetracyclin antibiotics. When a genetic region is disrupted by integration into the antibiotic resistance conferring genetic region say the tetracyclin of pBR322, the gene function is lost.This in turn helps in the selection of recombinant colonies as the recombinant colonies would now only grow in ampicillin containing medium because now it is sensitive to tetracyclin.
Regards