Ana based on

Dear student,The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. When a foreign DNA is ligated at the BamH I site of tetracycline resistance gene in the vector pBR322, the recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA.Now they will grow in ampicillin and not tetracycline.Option d. Regards.