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Animal cell cultures are grown in co2 incubator than regular BODS. why?

dear student 
incubators are generally used to control the environment such that each culture gets the optimum condition for its growth in terms of temperature, light, humidity, oxygen concentration etc etc. some cultures do need a CO2 incubator and some don't. it really depends on the cell type we are culturing. CO2 incubators maintain CO2 level at 5%. moreover, using incubators is much more convenient as they are automated.
Regards

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A CO2 incubator is used to culture cells to provide it with the optimum temperature, moisture (sterile environment) and to maintain optimum pH. When the media contains carbonate buffer, the CO2 gas from the cylinder is let into the incubator in such a way that the pH remains constant.. I hope you remember the equation where H2O + CO2 = H2CO3 exist in equilibrium. The pH is controlled on the same principle..
U can test this by keeping media inside the CO2 incubator and altering the CO2 levels..there will be frequent changes in the media color which indicates the change in pH (remember media contains an indicator which helps us detect the change in pH)..!!
HOPE IT WILL HELP YOU........
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