Describe any vector less method of introducing the rDNA into a competent host cell?
Vector less gene transfer can be done by Micro injection, Electroporation & Gene gun methods.
1. Micro injection is the process of introducing foreign DNA into the host cell by injecting the DNA directly into the nucleus by using micro needle or micro pipette.
2. Electroporation is the process by which transient holes are produced in the plasma membrane of the host cell to facilitate entry of foreign DNA.
3. Gene gun is the technique of bombarding micro projectiles coated with the foreign DNA with great velocity into the target cell.