Describe the biochemical reactions of DNA ligase Enzyme with example.
Solution:
Ligase enzyme covalently joins nicks in the fragments of double-stranded DNA by catalyzing the formation of a phosphodiester bond between two nucleotides. The joining of polynucleotides with the help of DNA ligase is necessary for DNA repair, recombination, and DNA replication which requires the joining of small, ssDNA fragments known as Okazaki fragments.
Ligase enzyme covalently joins nicks in the fragments of double-stranded DNA by catalyzing the formation of a phosphodiester bond between two nucleotides. The joining of polynucleotides with the help of DNA ligase is necessary for DNA repair, recombination, and DNA replication which requires the joining of small, ssDNA fragments known as Okazaki fragments.