Differentiate clonning vector and expression vector - Biology - Molecular Basis of Inheritance

Question

Differentiate clonning vector and expression vector

Namita Bisht · Accepted Answer

Solution:

Characteristics
Cloning Vector
Expression vector

Definition
It can be described as the small DNA fragment that is
responsible for maintaining stability inside the host cell It also
leads to creation of multiple gene copies of interest inside the
host cell
It can be described as an circular extra chromosomal,
double-stranded DNA It also has self-replicating properties

Features
It is made up of an ori, restriction sites, reporter gene and a
specific antibiotic resistance site
It is made up of enhancers, promoters, termination sequences,
transcription and translation initiation site and more

Types
Plasmids, YACs, BACs, cosmids and more
Plasmid

Use
It is used to introduce foreign gene into a host
It is used to express the introduced gene by synthesizing a
relevant protein

Characteristics	Cloning Vector	Expression vector
Definition	It can be described as the small DNA fragment that is responsible for maintaining stability inside the host cell. It also leads to creation of multiple gene copies of interest inside the host cell.	It can be described as an circular extra chromosomal, double-stranded DNA. It also has self-replicating properties.
Features	It is made up of an ori, restriction sites, reporter gene and a specific antibiotic resistance site.	It is made up of enhancers, promoters, termination sequences, transcription and translation initiation site and more.
Types	Plasmids, YACs, BACs, cosmids and more.	Plasmid
Use	It is used to introduce foreign gene into a host.	It is used to express the introduced gene by synthesizing a relevant protein.

Differentiate clonning vector and expression vector.