Distinguish between (a) isoenzyme and coenzyme(b)apoenzyme and coenzyme(c)enzyme and coenzyme?

Many enzymes require an additional small molecule, known as a cofactor to aid with catalytic activity. A cofactor is a non-protein molecule that carries out chemical reactions that cannot be performed by the standard 20 amino acids. Cofactors can be either inorganic molecules (metals) or small organic molecules (coenzymes).

Cofactors, mostly metal ions or coenzyme, are inorganic and organic chemicals that function in reactions of enzymes. Coenzymes are organic molecules that are nonproteins and mostly derivatives of vitamins soluble in water by phosphorylation; they bind apoenzyme protein molecule to produce active holoenzyme.

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Difference # Apo-Enzyme:

1. It is protein part of the holoenzyme or con­jugate enzyme.

2. It is large in size.

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3. An apoenzyme is specific for an enzyme.

4. It takes part in only catalytic activity of the enzyme.

5. Apoenzyme does not help in group transfer.

6. Apoenzyme is thermolabile.

Difference # Co-Enzyme:

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1. Coenzyme is the non-protein organic group which gets attached to the apoenzyme to form holoenzyme or conjugate enzyme.

2. It is small in size.

3. A coenzyme can function as a cofactor for a number of enzymes carrying out that particular type of reaction.

4. It helps in removing a product of chemical reaction besides bringing contact between the substrate and the enzyme.

5. Coenzyme takes part in group transfer.

6. Coenzyme is heat stable.

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Difference between apoenzyme and coenzyme

 

Apoenzyyme

Coenzyme

1.

It is the protein part of the enzyme composed of only amino acids.

It is the non-protein organic part of the enzyme which is attached to the apoenzyme  to form conjugate protein.

2.

It is heat sensitive i.e. Thermolabile.

It is heat stable.

3.

It is larger in size.

It is smaller in size.

4.

It is specific for an enzyme.

It can function as a cofactor for a number of enzymes carrying out specific functions.

5.

It is responsible for catalytic activity of the enzyme.

It takes part in the removal of the products of the reaction.

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