draw a labelles diagram of pbr322 explain the following a origin of replication b cloning vectors - Biology - Biotechnology Principles and Processes

Question

draw a labelles diagram of pbr322 explain the following a origin of
replication b cloning vectors c selectable markers

Decoder_6 · Accepted Answer

Dear student,
This is the structure of pBR322 ( Cloning vector)

<img alt="" src=
"https://s3mn%20mnimgs%20com/img/shared/content_ck_images/ck_5c42a990663cf%20jpg">
Cloning vector-
It is used to selection of recombinants from non-recombinants,
there are different cloning sites are there
Bam H1- site for tetracycline
Pvu I- site for ampicillin
Pst I- site for ampicillin

Ori- Origin of replication is the site where
replication starts and any piece of DNA, when linked to this
sequence, can replicate

Selectable marker- Ori is a
vector which requires a selectable marker to identify and
eliminating nontransformants
Genes encoding resistance to antibiotics such as ampicillin and
tetracycline

Regards
Meritnation

draw a labelles diagram of pbr322 explain the following. a. origin of replication. b. cloning vectors c. selectable markers