Experts, Please see this question once, and have a look at the answer given by the authors. In the highlighted line, they have written that non-transformants will grow in amp and tet mediums. First of all clear my confusion with the terms, recombinants and Transformants. 
Also, acc to NCERT, non-transformant  E coli has got no resistance to any of the antibiotics, so how can it grow in both the mediums?
So, is the answer wrong or what? Please clarify my all the doubts and don't ask me to post the question in different threads. 
It's a single question only.
  

 
Experts, Please see this question once, and have a look at the answer given by the authors. In the highlighted line, they have written that non-transformants will grow in amp and tet mediums. First of all clear my confusion with the terms, recombinants and Transformants.  Also, acc to NCERT, non-transformant  E coli has got no resistance to any of the antibiotics, so how can it grow in both the mediums? So, is the answer wrong or what? Please clarify my all the doubts and don't ask me to post the question in different threads.  It's a single question only.      (a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it. (b) What does the ampicillin resistant gene act as in the above case? [CBSE Delhi 2013] [HOTSI (a) E. coli bearing transferred recombinant DNA are first grown on ampicillin containing medium and then transferred on to a medium containing tetracycline. "Ihe transformants will grow only in ampicillin containing medium and not in tetracycline containing medium. The non-transformants, on the other hand, will grow in both the mediums. (b) Ampicillin resistant gene acts as a selectable marker and helps in selecting the transformants.

  • -1
ok sir
  • 0
What are you looking for?