Explain the effect of pH and temperature on enzyme linked reactions.
Dear Student,
An enzyme works best at it's optimum temperature and pH range. At a lower pH or temperature, the enzyme does not works at it's optimum level hence it is not able to increase the rate of reaction efficiently. Whereas at a higher temperature or pH level the enzyme may get denatured, thus the enzyme would not be able to play any significant role in that particular enzyme linked reaction.
Effect of pH on enzymes:
Enzymes are active within a narrow range i.e. between 5 to 9 pH. Some factors influence pH in which the reaction takes place. They are as follows:
1. Ionisation of the substrate
2. State of amino acid residues involved in catalytic activity of the enzyme.
3. Binding of substrate to enzyme.
4. Variation in protein structure.
The graph of pH against reaction rate is a bell shaped curve.
Effect of temperature on enzymes:
Temperature influences enzyme in two ways. viz. Direct influence or by denaturation of enzymes. If the temperature is too low, enzyme activity will be slow. As it reaches in the optimum range of temperature, rate of reaction will increase. But if the temperature increases above optimum level, thermal deactivation of enzymes occurs. For most of the mammals, optimum temperature is 37°C. Deactivation of enzymes may be reversible or irreversible.
Regards
An enzyme works best at it's optimum temperature and pH range. At a lower pH or temperature, the enzyme does not works at it's optimum level hence it is not able to increase the rate of reaction efficiently. Whereas at a higher temperature or pH level the enzyme may get denatured, thus the enzyme would not be able to play any significant role in that particular enzyme linked reaction.
Effect of pH on enzymes:
Enzymes are active within a narrow range i.e. between 5 to 9 pH. Some factors influence pH in which the reaction takes place. They are as follows:
1. Ionisation of the substrate
2. State of amino acid residues involved in catalytic activity of the enzyme.
3. Binding of substrate to enzyme.
4. Variation in protein structure.
The graph of pH against reaction rate is a bell shaped curve.
Effect of temperature on enzymes:
Temperature influences enzyme in two ways. viz. Direct influence or by denaturation of enzymes. If the temperature is too low, enzyme activity will be slow. As it reaches in the optimum range of temperature, rate of reaction will increase. But if the temperature increases above optimum level, thermal deactivation of enzymes occurs. For most of the mammals, optimum temperature is 37°C. Deactivation of enzymes may be reversible or irreversible.
Regards