How are restrictions enzyme are different from recombinase enzymes? (2/3marker).
Solution:
Restriction endonucleases are the special type of endonucleases that identify and cleave only specific sequences (recognition sequences) and these sequences are palindromic sequences (sequences of bases that would read the same if their complementary bases are read with the same orientation). For example, EcoRI, Hind III, PvuI , BamHI, etc.
An example of a palindromic sequence is given below.
5' GAATTC 3'
3' CTTAAG 5'
It is the recognition sequence for EcoRI.
Restriction endonucleases are the special type of endonucleases that identify and cleave only specific sequences (recognition sequences) and these sequences are palindromic sequences (sequences of bases that would read the same if their complementary bases are read with the same orientation). For example, EcoRI, Hind III, PvuI , BamHI, etc.
An example of a palindromic sequence is given below.
5' GAATTC 3'
3' CTTAAG 5'
It is the recognition sequence for EcoRI.
Recombinase enzymes are involved in genetic recombination. Some examples are DNA recombinase active during the pachytene stage of prophase I of meiosis I), RecA, and Cre recombinase.