How is action if normal endonuclease enzyme different from that of restriction endonucleases?
Dear student
Restriction endonucleases are the enzymes produced by bacteria that recognize and cleave at a specific sequence in the polynucleotide chain and not the ends defined by 3-OH or 5’ phosphoryl groups.
Restriction endonucleases are the enzymes produced by bacteria that recognize and cleave at a specific sequence in the polynucleotide chain and not the ends defined by 3-OH or 5’ phosphoryl groups.
-
Example: EcoR1 cleaves site
-
Restriction enzymes cut a little away from the centre of pallindrome site, but between the same two bases on the opposite strands.
-
As a result, overhangs (called sticky ends) are generated on each strand.
-
Sticky ends form hydrogen bonds with their complementary counterparts with help of DNA ligases.
-
All these processes form the basis of RDT.
On other hand, There are endonucleases in DNA that are responsible for removing a wrongly matched base or damage caused in the middle of a polynucleotide chain. Most of such endonucleases are involved in repair of DNA.
Endonucleases Cut the DNA by cleaving the phosphodiester bonds within the polynucleotide chain of DNA.
Regards