How probe sequence is determined in autoradiography molecular diagnosis

Dear student,
In case of autoradiography, probe sequences are determined by application of radiolabeled ligands in the sections in inspection. Usually ligands are marked with ³H or ¹²⁵I isotopes are used, although in cases of RNA or DNA viral sequences, ³²P, ³³P or ³⁵S probes are also useed. Via marking and detecting the radioactive emissions from the probes attached, the sequence patterns and the functional patterns are detected.

Regards.