If in a linear DNA, the sequence GAATTC appears thrice, how many restriction fragments will be produced on using Eco R1 as endonuclease?
(1) 2
(2) 3
(3) 4
(4) Cannot be predicted
Answer is (3).How???????

Dear student,
Please find below the solution to the asked query

The correct option is (3).
The ECoR1 cuts the  DNA between bases G and A only when the sequence is GAATTC. When the GAATTC is present thrice in linear DNA, four fragments are generated. This can be illustrated as:

Hope this information will clear your doubts about the fragments produced on using EcoR1 as endonuclease, when GAATTC is thrice in linear DNA.
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Let's take it as a DNA sequence n d exclamatory marks as cuts...  
If we put first cut it divides into two parts n the second part is common between first n second cut .. Similarly one part is common between cuts... So total 4 fragments are obtained.
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