In the plasmid pBR 322 there are two antibiotic resistance genes called ampicillin resistance gene ( ampR) and tetracylin resistance gene called (tetR ) . In the recognition site of ( ampR ) Pst 1 or Pvu 1 , when the ligation of a alien DNA is carried out , why does the gene ampR become non-functional ? PLEASE DON'T GIVE ME AN EXTRACT FROM A TEXTBOOK . PLEASE EXPLAIN THIS IN SIMPLE WORDS .

  • 0
What are you looking for?