in the RNAi mechanism silencing of the mRNA forms due to the binding of RISC with mRNA .my doubt from where the protein comes for the formation of RISC
RNA silencing occurs due to complementary double stranded RNA molecule which binds to and prevents translation of the mRNA. The source of this dsRNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) which replicate through an RNA intermediate.