kindly give a schematic representation of the working of elisa technique showing the use of P V C,serumetc - Biology - Biotechnology Principles and Processes

Question

kindly give a schematic representation of the working of elisa
technique showing the use of P V C,serumetc

Mushtaq Ahmed · Accepted Answer

<img alt="" src=
"https://s3mn%20mnimgs%20com/img/shared/discuss_editlive/2734998/2012_10_05_12_21_53/ckymmver_4721390490682280631%20png">
ELISA is a biochemical technique used mainly in
immunology to detect the presence of an antibody or an antigen like
drug or poison in a sample 
There are two main variations on this method: you can determine how
much antibody is in a sample, or you can determine how muchantigen
is bound by an antibody
Procedure for ELISA technique:
In general;
An unknown amount of the antigen is affixed to the surface of
the microtitre plates
A specific primary antibody is washed over
the surface so that it can bind to the antigen
The secondary antibody that is linked to an
enzyme such as peroxidase is added in the wells and
finally
A substance such as chromogenic substance is
added that enzyme can convert to some detectable signal The change
in the colour of the sample in the wells indicates the positive
result

kindly give a schematic representation of the working of elisa technique showing the use of P.V.C,serumetc