Name any 2 techniques that serve the purpose of diagnosis of bacterial/viral human diseases
Dear student.
The techniques are.
1. PCR: PCR ( Polymerase Chain Reaction) is a molecular technique that basically helps in amplifying the specific section of DNA to many times with the use of specific primers. This technique has various applications among which molecular diagnosis is one. PCR by amplifying the DNA to many times helps in detection of even minute quantities of nucleic acids( DNA ) easily. So also helps in early and highly sensitive detection of disease causing agents in the body by amplifying their specific DNA.
For example:
PCR helps in detection of infectious agents by virtue of specific genes. It helps in early detection of HIV (causing AIDS), even before the onset of disease. The specific primers to the targeted sequences in the DNA of a virus are used to amplify the viruses nucleic acid after it has been extracted from the blood. Then, the amplified viral DNA can be quantified.
PCR is also used to perform the diagnosis of cancers like leukemia and lymphomas. PCR, in this case, works by detecting translocation, gene mutation in genomic DNA of malignant cells of suspected cancer patients. PCR can amplify a specific DNA fragment with mutation by using specific primers and the result of PCR can be then detected on the gel with electrophoresis.
2. RDT: RDT refers to Recombinant DNA Technology. RDT is the process of creation of chimeric DNA molecules or recombinant DNA molecules which contain DNA from different organisms.
Regards.
The techniques are.
1. PCR: PCR ( Polymerase Chain Reaction) is a molecular technique that basically helps in amplifying the specific section of DNA to many times with the use of specific primers. This technique has various applications among which molecular diagnosis is one. PCR by amplifying the DNA to many times helps in detection of even minute quantities of nucleic acids( DNA ) easily. So also helps in early and highly sensitive detection of disease causing agents in the body by amplifying their specific DNA.
For example:
PCR helps in detection of infectious agents by virtue of specific genes. It helps in early detection of HIV (causing AIDS), even before the onset of disease. The specific primers to the targeted sequences in the DNA of a virus are used to amplify the viruses nucleic acid after it has been extracted from the blood. Then, the amplified viral DNA can be quantified.
PCR is also used to perform the diagnosis of cancers like leukemia and lymphomas. PCR, in this case, works by detecting translocation, gene mutation in genomic DNA of malignant cells of suspected cancer patients. PCR can amplify a specific DNA fragment with mutation by using specific primers and the result of PCR can be then detected on the gel with electrophoresis.
2. RDT: RDT refers to Recombinant DNA Technology. RDT is the process of creation of chimeric DNA molecules or recombinant DNA molecules which contain DNA from different organisms.
Regards.