Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid

Alkaline phosphatase is purified from either E. Coli or higher organisms (e.g. calf intestine). It is used for removal of 5′-phosphate groups from nucleic acids in order to prevent re circularization of DNA vectors in cloning experiments. Alkaline phosphatase does not hydrolyze phosphodiester bonds.

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