Observe the guven sequence of nitrogenous bases on a DNA fragment and answer the following question -
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
(a) Name a restriction enzyme which can recognize this DNA sequence.
(b) Write the sequence after digestion
(c) Why are the ends generated after digestion called sticky ends?
Dear student.
Please find the solution below.
1. The answer is EcoRI. The recognition sequence of EcoRI enzyme is 5' – GAATTC – 3'.
2. The sequence will be:
a. 5' -CAG- 3' and 5' -AATTCTTA- 3'
b. 3' -GTCTTAA- 5' and 3' -GAAT- 5'
3. They are called as stick ends because they form hydrogen bonds with their complementary counterparts with help of DNA ligases.
Regards.
Please find the solution below.
1. The answer is EcoRI. The recognition sequence of EcoRI enzyme is 5' – GAATTC – 3'.
2. The sequence will be:
a. 5' -CAG- 3' and 5' -AATTCTTA- 3'
b. 3' -GTCTTAA- 5' and 3' -GAAT- 5'
3. They are called as stick ends because they form hydrogen bonds with their complementary counterparts with help of DNA ligases.
Regards.