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Plant part used for tissue culture is sterilized by

Dear Student,
The plant part used for tissue culture is sterilised by chemicals and disinfectant like sodium or calcium hypochlorite.explants and stored and developed in the sterile condition under the laminar hood.
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Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as Petri dishes or flasks in a growth room with controlled temperature and light intensity........
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HEPA stands for High-efficiency particulate air, also known as high-efficiency particulate absorbing and high-efficiency particulate arrestance........
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