please explain what is mendels experiment  in detail????????


 

Gregor Johann Mendel (1822 – 1884) was the first to carryout the study on the transmission of characteristics from the parents to the offsprings. He proposed that heredity is controlled by factors, which are now believed to be segments of chromosomes or genes.

Mendel’s Experiments

Mendel performed experiments on a garden pea (Pisum sativum) with different visible contrasting characters. He worked on the following seven traits of garden pea:

S. No.

Character

Dominant

Recessive

1

Stem height

Tall

Dwarf

2

Flower colour

Violet

White

3

Flower position

Axial

Terminal

4

Pod shape

Inflated

Constricted

5

Pod colour

Green

Yellow

6

Seed shape

Round

Wrinkled

7

Seed colour

Yellow

Green

  • True breeding pea lines were obtained by continuous self pollination for several generations.
  • Fourteen true breeding pea lines were selected as pairs, which were similar except for one character with contrasting traits.
  • Artificial cross pollination (hybridisation) was performed on such varieties to obtain first hybrid generation known as the first filial progeny or F1.
  • After hybridisation, the F1 generation so obtained resembled only one of its parents (say, all tall; no dwarf).
  • When 2 plants from F1 generation were self pollinated, the second filial progeny or F2 generation was obtained.
  • Revival of unexpressed trait (dwarf) was observed in some F2 progeny. Both traits, tall and dwarf, were expressed in F2 in ratio 3:1.
  • Mendel proposed that something is being passed unchanged from generation to generation. He called these things as ‘factors’ (presently called genes).
  • Factors contain and carry hereditary information.
  • Alleles − Slightly different form of same factor

Two alleles code for a pair of two contrasting traits. (e.g., tall and dwarf)

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 In one experiment, Mendel cross-pollinated smooth yellow pea plants with wrinkly green peas. (The organisms that are used as the original mating in an experiment are called the parental generation and are marked by P in science textbooks). Every single pea in the first generation crop (marked as f1) was as yellow and as round as was the yellow, round parent. Somehow, yellow completely dominated green and round dominated wrinkly. 

Mendel learned from this that there are two kinds of traits - dominant and recessive. In this case, the dominant traits are the yellow color and the round shape since they show up at the expense of the green color and the wrinkly shape. He also learned that the inheritance of each trait is determined by "units" or "factors" - now called genes. 

Now he went on with his experiment and planted seeds from the all-yellow, all-round crop, achieved from the parent generation, and self-pollinated the grown up plants. The results led to some surprises. Most of the second generation (marked as f2) of peas were yellow and smooth, but some were green or wrinkly. Mendel repeated his experiment many times and the f2 generation consistently had a 3:1 ratio of yellow to green and round to wrinkly. How did Mendel explain his results? 

Interactive pea plant experiment
Interactive pea experiment where you can breed your own hybrid pea plants!


As mentioned above, Mendel postulated that there are dominant and recessive traits in heredity. 
In his experiment Mendel marked with capital letters dominant traits and with small letters recessive traits. 

    Y = dominant yellow color
    g = recessive green color
    R = dominant round shape
    w = recessive wrinkly shape

When the f1 plants breed, each has an equal chance of passing on either Y or g units to each offspring (f2 generation), and the same is with R and w. 

In the pollination process the offsprings get one trait unit (gene) from every parent; in the case of color the possible combinations aregY Yg YY gg. Three combinations from the four - gY Yg YY - will give yellow offsprings since all of them possess at least one Y dominant unit. Only one combination from the four - gg - will give green offsprings since it has two recessive units.

Because, in order to show-up, a dominant trait needs only one trait unit from one of the parents, and the recessive one needs two, from both parents, in order to prevail, that is the reason why the ratio between occurrences of dominant traits and recessive traits is 3:1. The same explanation applies to the shape traits.

Mendel came to three important conclusions from these experimental results: 

  • That the inheritance of each trait is determined by "units" or "factors" (now called genes) that are passed on to descendents unchanged
     
  • That an individual inherits one such unit from each parent for each trait
     
  • That a trait may not show up in an individual but can still be passed on to the next generation. 
     
Repeat Mendel’s Pea Plant Experiment


For his Experiments, Mendel chose pea plants because they had some distinctive measurable traits and being easy to breed with a short breeding period - some cultivars reach maturity about 60 days after planting.

He measured seven pea characteristics:

  • Color and smoothness of the seeds - grey and round or white and wrinkled
  • Color of the cotyledons (part of the embryo within the seed) - yellow or green
  • Color of the flowers - white or violet
  • Shape of the pods - full or constricted
  • Color of unripe pods - yellow or green
  • Position of flowers and pods on the stems
  • Height of the plants - short or tall

Mendel’s actual experiments are more complicated than described above. For example, in 1936, the statistician R.A. Fisher used a chi-square test to analyze Mendel's data and concluded that Mendel's results with the predicted ratios were far too perfect, indicating that adjustments (intentional or unconscious) had been made to the data to make the observations fit the hypothesis. Later authors have claimed Fisher's analysis was flawed, proposing various statistical and botanical explanations for Mendel's numbers. It is also possible that Mendel's results are "too good" merely because he reported the best subset of his data — Mendel mentioned in his paper that the data was from a subset of his experiments (Wikipedia).
Fairbanks, D. J.; Rytting, B. (01 May 2001). "Mendelian Controversies: A Botanical and Historical Review". American Journal of Botany 88 (5): 737.

Before you begin, consult the link section of this page and the further resources provided. The list begins with basics and ends with more advanced resources including Mendel's writings himself. Ensure that you understand the basic principals. Surf the web further and consult your local library, your teachers and other knowledgeable adults and experts. 

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