Pls Explain these Paragraph In detail And explain how does the ori of vector Impact on Desired gene?

Pls Explain these Paragraph In detail And explain how does the ori of vector Impact on Desired gene? 1) The introduced piece of DNA does not replicate in the host unless it is integrated with the chromosome of host. For getting replicated, the foreign DNA must integrate into the host DNA sequence having 'origin of replication'. When this integration occurs, foreign DNA is replicated and many copies are formed. This process is called cloning (the process of formation of multiple identical copies of DNA). 2)lf foreign DNA is linked to these vectors, then it is multiplied to the number equal to the copy number of vector. Replication starts from ori. Any fragment of DNA when linked to ori can be made to replicate. With the help of this, the genetic engineer may control copy number of the recombinant DNA. To recover a high number, suitable origin of replication must be chosen. If Vectors Just Transfers The. Desirable DNA into The Host. How Does The Vectors Ori Impact on Multiplication if Desired gene?

Dear student,
The paragraph means that replication of introduced DNA in the host does not takes place unless it is integrated into the host genome.

To start the replication, the foreign DNA must be integrated with Origin of replication thus it helps in replication which is called as Cloning.

Hence when a large number of DNA is required it iuse a suitable Origin of Replication.

The vector's Ori has an impact on multiplcation on desired gene as Ori has a major role to play in replication and to recover a large number of DNA copies suitable Ori should be opted.

Hope this information will clear your doubts about topic.

If you have any more doubts just ask here on the forum and our experts will try to help you out as soon as possible.

Regards

 

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