Plz ans question 5
Dear Akash,
The gene of interest can be multiplied in vitro using a technique known as PCR. In other words, In vitro PCR (polymerase chain reaction) is used to increase the copy of gene.
Three steps are involved in this technique.
Denaturation:- It involves the separation of double stranded DNA into single strand at higher temperature
Annealing:- This involves the attachment of primer to the single stranded DNA at lower temperature.
Extension:- At higher temperature the annealed primer extends the length of new strand of DNA by adding nucleotide to the 3'OH end of the primer.
All the best
Regards
The gene of interest can be multiplied in vitro using a technique known as PCR. In other words, In vitro PCR (polymerase chain reaction) is used to increase the copy of gene.
Three steps are involved in this technique.
Denaturation:- It involves the separation of double stranded DNA into single strand at higher temperature
Annealing:- This involves the attachment of primer to the single stranded DNA at lower temperature.
Extension:- At higher temperature the annealed primer extends the length of new strand of DNA by adding nucleotide to the 3'OH end of the primer.
All the best
Regards