Plz ans question 5

Dear Akash,

The gene of interest can be multiplied in vitro using a technique known as PCR. In other words, In vitro PCR (polymerase chain reaction) is used to increase the copy of gene.

Three steps are involved in this technique.
Denaturation:- It involves the separation of double stranded DNA into single strand at higher temperature
Annealing:- This involves the attachment of primer to the single stranded DNA at lower temperature.
Extension:- At higher temperature the annealed primer extends the length of new strand of DNA by adding nucleotide to the 3'OH end of the primer.

All the best

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