Q). What does this mean?
because of the requirement of the origin of replication that a piece of DNA if needed to be propagated during recombinant DNA procedures, requires a vector. The vectors provide the origin of replication.

Dear student

Please refer the solution below for the said query


It means that an orgin of replication is needed to produce multiple copies of a desired gene (a piece of DNA) during recombinant DNA technology. The origin  of replication is a region where replication of DNA is started by the enzymes. 

The desired gene (a piece of DNA) does not have its own origin of replication. Hence, desired gene is ligated with vector DNA like plasmid of bacteria containing origin of replication for producing its multiple copies in any host organism (say E.coli). 



Hope this information clears your doubt about the topic.

Regards

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