Steps in formation of recombinant DNA bybaction of restriction endonuclease enzyme- EcoRI
1. isolation of genetic material i.e. dna.
2. cutting of dna at specific locations by restriction endonucleases
3. copying of dna by polymerase chain reaction (pcr)
4. insertion of recombinant dna into competent cells
5. obtaining the foriegn gene product
6. downstream processing
2. cutting of dna at specific locations by restriction endonucleases
3. copying of dna by polymerase chain reaction (pcr)
4. insertion of recombinant dna into competent cells
5. obtaining the foriegn gene product
6. downstream processing