Test on protrin in differen pulses

Dear Student

Biuret Test

Biuret solution is used to identify the presence of protein. It is a blue solution that contains copper sulphate and sodium hydroxide, when it reacts with protein, will change color to pink-purple or violet.

Procedure:
Take a small quantity of the food items to be tested(pulses in this case)
Grind/mash/paste/powder the food item (pulse)
Put the food item into a test tube.
Add 10 drops of water to the test tube.
Add two drops of copper sulphate solution to the test tube.
Add 10 drops of sodium hydroxide solution and shake the test tube.
Observe the colour of the mixture.
Observation:
Violet colour indicates the presence of proteins in the tested food item (pulse).

Regards

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