The technique of osmotic pressure for determination of molar mass of solutes is particularly useful for biomolecules as they are generally not stable at high and polymers have poor solubility. PLEASE ELABORATE THESE LINES AND GIVE REASON THAT why are( i)(a)biomolecules are generally not stable at high temperature and(b) polymers have poor solubility (ii)how Osmosis pressure is affected by this property ¤ 《《please don't send any link and Don't Copy the answer》》

Dear Student

Biomolecules are generally made up of amides and glycosidic linkages. Amides are the bonds between NH and carbonyl CO (like in DNA) and glycosidic bond is between carbon and carbonyl CO (like in glucose), these bond are weak at high temperature and gets rupture in presence of heat.

Also, solubility of a substance or a specie decreases if the molecular mass is increases. Polymers due to a very high molecular mass have low solubility.

Thus, unlike molality in colligative properties molarity is used instead in calculation of the osmotic pressure, which can be done at room temperature and instead of calculating the weight or mole fraction of solute (for molality), we deal with the concentration of the solution (for molarity).

Hope this helps!


  • 1
What are you looking for?