to prepare temporary staned mount of glycerine of striated muscles from cockroach leg

To prepare a temporary stained mount of striated muscle:

1. Place a preserved cockroach on dissecting tray.

2. Cut open the thigh region and remove a small piece of muscle from this region. Put it on a slide and tease with a needle to get thinnest possible fibre.

3. Wash with water 2 - 3 times in a petridish.

4. Stain with methylene blue, bot out excess water and mount with the help of glycerine.