What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
The function of these sites is to provide a place for a restriction enzyme to cut, so that when we are cloning a vector we can easily insert a gene of our interest by cutting the vector at restriction site and making space for the new gene.