What is BamH1??? For what is it used?
Bam HI is a type of restriction endonuclease II that recognizes the short sequences of DNA and cleaves them specifically at a target site.
BAM HI, in its dimer form, recognizes and cleaves the DNA palindromic sequence GGATCC in the presence of Mg2+ generating a 4 base 5' extensions.
Lets take the example of the following DNA sequence.
Bam HI acts on the above DNA sequence and cuts it into 2 fragments as shown below
BamHI (from Bacillus amyloli) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 b.p.) of DNA and specifically cleaving them at a target site. This exhibit focuses on the structure-function relations of BamHI as described by Newman, et al. (1995)
Each BamHI subunit makes the majority of its backbone contacts with the phosphates of a DNA half site but base pair contacts are made between each BamHI subunit and nitrogenous bases in the major groove of the opposite DNA half site.