what is the principle of seperation of DNA fragmemys by gel electrophoration?

Electrophoresis is a technique which is used to purify and separate proteins and nucleic acid molecules, which differ in their size, charge and conformation. Gel is used to electrophoresed proteins and  nucleic acids, in this case DNA, hence the name gel electrophoresis.
Principle of electrophoresis for DNA-  DNA molecules are separated according to their size in an agarose gel. DNA fragments are generally separated by the application of current or an electric field so that the negatively charged DNA molecules move through an agarose gel. The smaller molecules of DNA move at a faster rate than the larger one in the gel. 

  • 0

it is used in clinical chemistry to separate proteins by charge and/or size (dna is negatively charged) and inbiochemistryandmolecular biologyto separate a mixed population ofDNAandRNAfragments by length, to estimate the size ofDNAandRNAfragments or to separateproteinsby charge.[1]Nucleic acid molecules are separated by applying anelectric fieldto move the negatively charged molecules through a matrix ofagaroseor other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.[2]Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.

  • 0
What are you looking for?