what is the principle of seperation of DNA fragmemys by gel electrophoration?
Electrophoresis is a technique which is used to purify and separate proteins and nucleic acid molecules, which differ in their size, charge and conformation. Gel is used to electrophoresed proteins and nucleic acids, in this case DNA, hence the name gel electrophoresis.
Principle of electrophoresis for DNA- DNA molecules are separated according to their size in an agarose gel. DNA fragments are generally separated by the application of current or an electric field so that the negatively charged DNA molecules move through an agarose gel. The smaller molecules of DNA move at a faster rate than the larger one in the gel.
Principle of electrophoresis for DNA- DNA molecules are separated according to their size in an agarose gel. DNA fragments are generally separated by the application of current or an electric field so that the negatively charged DNA molecules move through an agarose gel. The smaller molecules of DNA move at a faster rate than the larger one in the gel.