Which vector is used to raise nematode pest resistant plant and increase tobacco production?

Dear student,
Nematode specific genes are introduced into tobacco plants using vector Agrobacterium tumefaciens for producing resistance in tobacco plant with nematode. The bacterium Agrobacterium tumefaciens is known to be natural vector capable of passing its DNA to plants and induce tumour by integrating its DNA with host genome. Following steps are involved for making tobacco plant resistant to infection by nematode:-
1) The gene in nematode which causes infection is isolated and incorporated into the tobacco plant with the help of  vector ​Agrobacterium tumefaciens.
2) The sense and anti sense mRNA produced are complementary to each other and base pairs with each other forming double stranded RNA.
3) This double stranded RNA causes RNA interference in tobacco plants thus neutralizes mRNA of nematode. Thus nematode is unable to live on tobacco plant and tobacco plant becomes resistant to nematode.

Regards
 

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