while doing PCR 'denaturation step is missed, How will be the process be affected?
Please find below the solution to the asked query
Denaturation is process in which double helical DNA unwinds at high temperature to produce single stranded DNA templates.
If the denaturation step is missed, the single stranded template needed for annealing of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) will not be produced. Hence, PCR reaction will not proceed further.
Hope this information will clear your doubts about the PCR.
Regards
Denaturation is process in which double helical DNA unwinds at high temperature to produce single stranded DNA templates.
If the denaturation step is missed, the single stranded template needed for annealing of primers (small chemically synthesised oligonucleotides that are complementary to the regions of DNA) will not be produced. Hence, PCR reaction will not proceed further.
Hope this information will clear your doubts about the PCR.
Regards