Why do we use chilled ethanol in purification of DNA
Dear student,
Chilled ethanol is used to increase the yield of DNA,this is due to that as temperature decreases,there is more and more precipitation.
Other reason is that at less temperature enzymes like DNAase is temperature sensitive and therefore DNAases are not active when temperature is low.Since these enzymes are involved in DNA lysis and degradation,reduction in this enzyme activity yields more DNA and helps in purification.
Hope this information will clear your doubts about topic.
If you have any more doubts just ask here on the forum and our experts will try to help you out as soon as possible.
Regards
Chilled ethanol is used to increase the yield of DNA,this is due to that as temperature decreases,there is more and more precipitation.
Other reason is that at less temperature enzymes like DNAase is temperature sensitive and therefore DNAases are not active when temperature is low.Since these enzymes are involved in DNA lysis and degradation,reduction in this enzyme activity yields more DNA and helps in purification.
Hope this information will clear your doubts about topic.
If you have any more doubts just ask here on the forum and our experts will try to help you out as soon as possible.
Regards