why is insersional inactivation better than inactivation of anitbiiotic to select transforments

In insertional inactivation, a foreign gene is inserted within -galactosidase gene, the enzyme -galactosidase gets  inactivated. Then the bacteria are grown on a chromogenic substrate. Non-recombinants will produce blue-coloured  colonies. Recombinants will produce colourless colonies. So, recombinants can be easily distinguished from  non-recombinants. 

While in antibiotic resistance,a  foreign DNA fragment is inserted within the tetR gene. Hence, tetracycline resistance is not present in the recombinants. So, recombinants will grow on the media containing ampicillin, but will die on media containing tetracycline. On the other hand, non-recombinants will grow on medium containing ampicillin as well as on medium  containing tetracycline. So, this method is comparatively difficult than insertional inactivation. 

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answer fast. i hav board exm, the day aftr tmrw

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