You have created a recombinant DNA molecule by ligating a gene to a plastic vector. By mistake your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go for transformation now ?

Dear student
Please find below the solution to the asked query

The correct term is plasmid vector.

The exonucleases are used to cut the linear DNA at free ends. Most likely the experiment will not be affected as recombinat DNA molecule formed by ligating a gene to a plasmid vector is a closed and circular without free ends.


Hope this information will clear your doubts about the topic.
Regards
 

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