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what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
When does DNA replication occur in prokaryotic cells?
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
Is enzymes or DNA bigger in molecular size?how do u know?
explain the structure of pBR322
What is BamH1??? For what is it used?
how to deliver genes to transform eukaryotic cells and force them to do what the bacteria or viruses want.
in plants, how is alien DNA introduced into the host cell?
meaning of biotechnology acc. to EFB?
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
what is cumbersome procedure ? and
what is chromogenic substeate ?
significance of genetic engineering
At which stage of meiosis a recombinant DNA is made?
significance of genetic engineering
What is genetic engineering?Explain Briefly the steps involved in this process?
What is the difference between recombinant and transformant?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
What's the difference between -galactosidase and Beta-galactosidase?
(b) Restriction enzymes and DNA
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
how we can identify non transformate from transformate based on insertional inactivation??????????
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
Full form of BAC, YAC and UTR
explain gel electrophoresis
Give example of molecular scissors?
eukaryotic cells have restriction endonucleases? Justify your answer.
notes on biotechnology
be the molar concentration of human DNA in a human cell? Consult your
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
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