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what is a heterologous host??
Define DNA spooling...?
what is the difference between recombinant and non - recombinant ?
What is the function of renette cells?
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
explain the process of elution?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
Is enzymes or DNA bigger in molecular size?how do u know?
in plants, how is alien DNA introduced into the host cell?
explain the structure of pBR322
What is BamH1??? For what is it used?
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
meaning of biotechnology acc. to EFB?
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
At which stage of meiosis a recombinant DNA is made?
significance of genetic engineering
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
What is genetic engineering?Explain Briefly the steps involved in this process?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
What's the difference between -galactosidase and Beta-galactosidase?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
name the specific components and the linkage btw them that form deoxyadenosine.
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
what are strain
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Why are restriction endonuclease so called?explain the role of molecular scissors in Recombinant DNA technology?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
how is gene Z used as a marker?
explain gel electrophoresis
Do eukaryotic cells have restriction endonucleases? Justify your answer.
How and why is the bacterium Thermus aquaticus employed in recombinant DNA technology? Explain.
notes on biotechnology
why is the introduction of genetically enginered lymphocyte into an ada deficiency patient not apermanent cure ? suggest a possible permanent cure
What is the difference between recombinant and transformant?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
what is biopiracy?....how has india been affected by it?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
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Syllabus
what is a heterologous host??
Define DNA spooling...?
what is the difference between recombinant and non - recombinant ?
What is the function of renette cells?
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
a)Linkage, mutation
b)Recombination, Linkage
c)Mutation, Recombination
d)Aneuploidy, Linkage
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
explain the process of elution?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
difference between transformant and non transformant
and pyrimidine bases. Also express the functions of the three types of RNA.
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
how many fragments will be produced from linear DNA and plasmidrespectively. explain..?Is enzymes or DNA bigger in molecular size?how do u know?
in plants, how is alien DNA introduced into the host cell?
explain the structure of pBR322
What is BamH1??? For what is it used?
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
meaning of biotechnology acc. to EFB?
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
(a) Draw schematic diagrams of segments of a vector and a foreign DNA with the sequence of nucleotides recognised by EcoRI.
(b) Draw the vector DNA segment of foreign DNA segments after the action of EcoRI and label the stickey end produceed.
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
A . Why this toxin does not kill the bacteria?
B.what type of changes occur in the gut of insects on consuming this protein?
C. How man has exploited this protein for his benefit?
Which one of an intron and an exon is the reminiscent of antiquity???
A agarose
B chitinase
C ethanol
D water
At which stage of meiosis a recombinant DNA is made?
significance of genetic engineering
significance of genetic engineering
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
What is genetic engineering?Explain Briefly the steps involved in this process?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
What's the difference between -galactosidase and Beta-galactosidase?
eukaryotic cell ?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
name the specific components and the linkage btw them that form deoxyadenosine.
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
what are strain
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Why are restriction endonuclease so called?explain the role of molecular scissors in Recombinant DNA technology?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
how is gene Z used as a marker?
explain gel electrophoresis
Do eukaryotic cells have restriction endonucleases? Justify your answer.
How and why is the bacterium Thermus aquaticus employed in recombinant DNA technology? Explain.
Can anyone explain this definition properly with regards to what the terms used mean?
notes on biotechnology
why is the introduction of genetically enginered lymphocyte into an ada deficiency patient not apermanent cure ? suggest a possible permanent cure
What is the difference between recombinant and transformant?
From the diagram of NCRT (of action of restriction endonuclease) give the direction of the both ends whereever 3 end or 5 end are required!
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
what is biopiracy?....how has india been affected by it?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?