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what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
in plants, how is alien DNA introduced into the host cell?
explain the structure of pBR322
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
What is BamH1??? For what is it used?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
meaning of biotechnology acc. to EFB?
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
When does DNA replication occur in prokaryotic cells?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
significance of genetic engineering
At which stage of meiosis a recombinant DNA is made?
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
What is genetic engineering?Explain Briefly the steps involved in this process?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
What's the difference between -galactosidase and Beta-galactosidase?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
how is gene Z used as a marker?
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
what is biopiracy?....how has india been affected by it?
explain gel electrophoresis
Do eukaryotic cells have restriction endonucleases? Justify your answer.
notes on biotechnology
how is artificial insulin produced by recombinant DNA technology?
desired gene is extracted from human cell then carried by vector plasmid this rdna is allow to multiply in host e.coli soinsolin is extracted by rdt.
What is the difference between recombinant and transformant?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
What do we mean by plasmid,host organism,alien DNA,palindrome sites and vectors in chapter biotechnology?
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
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Syllabus
what is a heterologous host??
and pyrimidine bases. Also express the functions of the three types of RNA.
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
in plants, how is alien DNA introduced into the host cell?
explain the structure of pBR322
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
What is BamH1??? For what is it used?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
how many fragments will be produced from linear DNA and plasmidrespectively. explain..?Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
meaning of biotechnology acc. to EFB?
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
When does DNA replication occur in prokaryotic cells?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
significance of genetic engineering
A) ATGCAT
B)ATCATC
C)AAAGGG
D)ATCCTA
PLEASE EXPLAIN THE REASON FOR THE ANSWER ALONG
At which stage of meiosis a recombinant DNA is made?
Q.131. Anucleic acid extracted from animal liver is loaded and run on agarose gel. After staining it shows following pattern
If the remaining sample is treated with RNAse and loaded in gel what result would you
(a)
(b)
(c)
(d)
significance of genetic engineering
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
What is genetic engineering?Explain Briefly the steps involved in this process?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
following questions.
In E. coli cloning vector pBR322, a foreign gene segment is to be introduced into the
ampR region. From the restriction enzymes given below, which one of you would
probably use and why? Bam HI, Sal I, Pvu II, Eco RI.
Highlight the reasons for why you could not choose the remaining restriction enzymes
for your work.
How will the insertion of this enzyme aid in recombinant DNA technological process?
What's the difference between -galactosidase and Beta-galactosidase?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
how is gene Z used as a marker?
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
the restriction fragment contains a gene whose recessive allele is Lethal the normal gene has restriction sites for the restriction enzyme PST 1 at sides 1 and 2. The recessive allele lacks restriction site 1. an individual who had a sister with the little treat is being tested to determine if he is a carrier of that allele indicate which of these band patterns would be produced on a gel if he is a carrier.
Give example of molecular scissors?
what is biopiracy?....how has india been affected by it?
explain gel electrophoresis
Do eukaryotic cells have restriction endonucleases? Justify your answer.
A . Why this toxin does not kill the bacteria?
B.what type of changes occur in the gut of insects on consuming this protein?
C. How man has exploited this protein for his benefit?
Can anyone explain this definition properly with regards to what the terms used mean?
notes on biotechnology
how is artificial insulin produced by recombinant DNA technology?
desired gene is extracted from human cell then carried by vector plasmid this rdna is allow to multiply in host e.coli soinsolin is extracted by rdt.
What is the difference between recombinant and transformant?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
What do we mean by plasmid,host organism,alien DNA,palindrome sites and vectors in chapter biotechnology?
which is the best book for hsc 12th ciass of biology
eukaryotic cell ?
what does non recombinant and non transformant mean?