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what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
explain the structure of pBR322
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
What is BamH1??? For what is it used?
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
in plants, how is alien DNA introduced into the host cell?
meaning of biotechnology acc. to EFB?
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
what do you mean by disarmed ?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
significance of genetic engineering
At which stage of meiosis a recombinant DNA is made?
1)Give structure of mature insuline molecule?
2)Give role of transgenic animals in production of biological product?
3)different methods to introduce recombinant DNA into host cell?
4)Explain advantages of geneticaly modiefied dna?
5)Explain how gene therphy can be used to treat ada deficiency?
6)Why foreign DNA and vector DNA are cut by same restriction endonucleus?
7)Give detail account of biotechnology application for production of pest resistant plants?
8)How is genetic material DNAisolated from cell in its pure form?
9)describe PCR?
10)explain how human insulin is produced using recombinant DNA technology?
11)what is palnotropic in DNA?
11)explain in brief 3 features that are required to facilitate cloning into a vector?
12) Why allien piece of DNA in an organism needs to be linked to ORI?why?
What is genetic engineering?Explain Briefly the steps involved in this process?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
When does DNA replication occur in prokaryotic cells?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
Observe the given sequence of nitrogenous bases on a DNA fragment and
What's the difference between -galactosidase and Beta-galactosidase?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
explain gel electrophoresis
what is the difference between vector and foreign DNA??
Do eukaryotic cells have restriction endonucleases? Justify your answer.
notes on biotechnology
What is the difference between recombinant and transformant?
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
what is the principle of seperation of DNA fragmemys by gel electrophoration?
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Syllabus
what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
how many fragments will be produced from linear DNA and plasmidrespectively. explain..?A . Why this toxin does not kill the bacteria?
B.what type of changes occur in the gut of insects on consuming this protein?
C. How man has exploited this protein for his benefit?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
difference between transformant and non transformant
and pyrimidine bases. Also express the functions of the three types of RNA.
What is "rop" in the cloning vector pBR322.....??
p;z explain:
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
explain the structure of pBR322
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
What is BamH1??? For what is it used?
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
in plants, how is alien DNA introduced into the host cell?
meaning of biotechnology acc. to EFB?
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
what do you mean by disarmed ?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
significance of genetic engineering
At which stage of meiosis a recombinant DNA is made?
following questions.
In E. coli cloning vector pBR322, a foreign gene segment is to be introduced into the
ampR region. From the restriction enzymes given below, which one of you would
probably use and why? Bam HI, Sal I, Pvu II, Eco RI.
Highlight the reasons for why you could not choose the remaining restriction enzymes
for your work.
How will the insertion of this enzyme aid in recombinant DNA technological process?
significance of genetic engineering
1)Give structure of mature insuline molecule?
2)Give role of transgenic animals in production of biological product?
3)different methods to introduce recombinant DNA into host cell?
4)Explain advantages of geneticaly modiefied dna?
5)Explain how gene therphy can be used to treat ada deficiency?
6)Why foreign DNA and vector DNA are cut by same restriction endonucleus?
7)Give detail account of biotechnology application for production of pest resistant plants?
8)How is genetic material DNAisolated from cell in its pure form?
9)describe PCR?
10)explain how human insulin is produced using recombinant DNA technology?
11)what is palnotropic in DNA?
11)explain in brief 3 features that are required to facilitate cloning into a vector?
12) Why allien piece of DNA in an organism needs to be linked to ORI?why?
What is genetic engineering?Explain Briefly the steps involved in this process?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
When does DNA replication occur in prokaryotic cells?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
Observe the given sequence of nitrogenous bases on a DNA fragment and
answer the following question –5´ – CAGAATTCTTA – 3´3´ – GTCTTAAGAAT – 5´(a) Name a restriction enzme which can recognise this DNA sequence.(b) Write the sequence after digestion.What's the difference between -galactosidase and Beta-galactosidase?
Explain briefly
(a) PCR
(b) Restriction enzymes and DNA
(c) Chitinase
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
explain gel electrophoresis
what is the difference between vector and foreign DNA??
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Can anyone explain this definition properly with regards to what the terms used mean?
(a) Draw schematic diagrams of segments of a vector and a foreign DNA with the sequence of nucleotides recognised by EcoRI.
(b) Draw the vector DNA segment of foreign DNA segments after the action of EcoRI and label the stickey end produceed.
notes on biotechnology
What is the difference between recombinant and transformant?
If in a linear DNA, the sequence GAATTC appears thrice, how many restriction fragments will be produced on using Eco R1 as endonuclease?
(1) 2
(2) 3
(3) 4
(4) Cannot be predicted
Answer is (3).How???????
What would be the molar concentration of human DNA in a human cell? Consult your teacher.
Experts, Please see this question once, and have a look at the answer given by the authors. In the highlighted line, they have written that non-transformants will grow in amp and tet mediums. First of all clear my confusion with the terms, recombinants and Transformants.
Also, acc to NCERT, non-transformant E coli has got no resistance to any of the antibiotics, so how can it grow in both the mediums?
So, is the answer wrong or what? Please clarify my all the doubts and don't ask me to post the question in different threads.
It's a single question only.
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
what is the principle of seperation of DNA fragmemys by gel electrophoration?