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what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
Define DNA spooling...?
What is the function of renette cells?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
who is father of biotechnology
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
explain the structure of pBR322
What is BamH1??? For what is it used?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
in plants, how is alien DNA introduced into the host cell?
meaning of biotechnology acc. to EFB?
Difference between transformant and recombinant?
how to deliver genes to transform eukaryotic cells and force them to do what the bacteria or viruses want.
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why antibiotics is a cumbersome procedure
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Provide one sentence information about plasmid with respect to its
Which one of an intron and an exon is the reminiscent of antiquity???
When does DNA replication occur in prokaryotic cells?
At which stage of meiosis a recombinant DNA is made?
significance of genetic engineering
significance of genetic engineering
What is genetic engineering?Explain Briefly the steps involved in this process?
what does non recombinant and non transformant mean?
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
what is bacteriophage.?
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
[Both of these have the ability to replicate within the bacterial cells independent of the chromosomal DNA.
Bacteriophages − Have high copy number (of genome) within the bacterial cell
Plasmids − May have 1 − 2 copy number to 15 − 100 copy number per cell]....................... i did ot get it.... pls anyone pls explain............
What's the difference between -galactosidase and Beta-galactosidase?
what is a Taq polymerase? from where it is obtained and what is its function?
(b) Restriction enzymes and DNA
name the specific components and the linkage btw them that form deoxyadenosine.
what are strain
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
explain gel electrophoresis
kindly give a schematic representation of the working of elisa technique showing the use of P.V.C,serumetc
eukaryotic cells have restriction endonucleases? Justify your answer.
notes on biotechnology
What is the difference between recombinant and transformant?
be the molar concentration of human DNA in a human cell? Consult your
which is the best book for hsc 12th ciass of biology
hey I think dat this lesson tells u about restriction endonuclease only and not about restriction enzymes. I think dat restriction endonuclease is one of the two restriction enzymes in which the other one adds methyl group to DNA. please check it and if neccesary do the appropriate changes.
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