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how is gene Z used as a marker?

  • Your friend Neha is correct!

 

  • The structural gene consist  of three gene (z, y and a)
  • Z -gene codes for beta-galactosidase, which hydrolyze lactose into Galactose and glucose.
  • Lac Z gene is a conditional non-selectable marker gene. LacZ gene has been a useful marker gene in many cell systems because it can be easily assayed and can form N-terminal translational fusions with other proteins.

 

@ Neha: Good Answer! Keep Posting!!

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may be if it is lac Z....

  • lacZ is a gene coding for Beta-Galactosidase (Beta-gal). Beta-gal cleaves a compound called X-galwhich is a type of ether. Beta-gal cleaves at the oxygen that connects both rings together. When X-gal is cleaved, you will produce a blue color. When you do cloning experiments...
  • you will ligate your DNA sequence into the lacZ gene. Well, when you break the lacZ gene, you cannot make a Bet-gal enzyme becasue the gene is "broken" or cut. So, X-gal is not cleaved, which gice you white colonies. You will pick the white colonies becasue they will have your vector transformed into the bacteria. The blue colonies have an intact lacZ gene and will produce Beta-gal, which in turn cleaves X-gal = blue colonies....
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