Pls provide the answer to these questions very urgently!!!
Dear Student
3.In the absence of antibiotic , there will be no pressure on recombinant to retain the plasmid ( containing the gene of interest). Since maintaining a high copy number of plasmids, a metabolic burden to the microbial cell is required otherwise it will thus tend to lose the plasmid.
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3.In the absence of antibiotic , there will be no pressure on recombinant to retain the plasmid ( containing the gene of interest). Since maintaining a high copy number of plasmids, a metabolic burden to the microbial cell is required otherwise it will thus tend to lose the plasmid.
For rest of the queries , please post them separately to get rapid assistance from our experts.
Regards