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Ans 4. The modification enzyme of restriction-modification system adds a methyl group to one or two bases within the recognition sequence of the restriction enzyme. Addition of a methyl group modifies the DNA sequence. As a result, the restriction enzymes fails to recognize and could not cut that DNA. This is how a bacterium modifies and protects its own DNA from cleavage by these restriction enzymes.
P.S. Kindly post the rest of the questions in a separate thread.
Regards
Ans 4. The modification enzyme of restriction-modification system adds a methyl group to one or two bases within the recognition sequence of the restriction enzyme. Addition of a methyl group modifies the DNA sequence. As a result, the restriction enzymes fails to recognize and could not cut that DNA. This is how a bacterium modifies and protects its own DNA from cleavage by these restriction enzymes.
P.S. Kindly post the rest of the questions in a separate thread.
Regards