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1) The new cells arise by the division of pre-existing cells through mitosis and meiosis.
2) In unicellular organisms, a single cell performs all the functions.
Example: E.coli, Paramecium
3) If a cell lacks a vacuole, it will be able to perform its functions properly. The function of vacuoles is to transport and sort nutrients, metabolites and waste products to the inside and outside of the cell. Lack of vacuole results in the accumulation of waste products inside the cell which may cause the death of the cell.
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