Solve this:
Q13. A scientist has cloned an 8 Kb fragment of a mouse gene into the Eco RI site of a vector of 6 Kb size. The cloned DNA has no other Eco RI site within. Digestions of the cloned DA is shown below.
Which one of the following sets of DNA fragments generated by digestion with both Eco RI and Bam HI as shown in (iii) is from the gene ?
Eco RI only Bam HI only ECoRI and BamHI
(A) 1 Kb and 4 Kb (B) 1Kb and 2.5 Kb
(C) 1 Kb and 3 Kb (D) 1 Kb and 3.5 Kb
Dear student
Please find below the solution to the asked query
The correct option is C.
Regard
Please find below the solution to the asked query
The correct option is C.
- When 8 Kb fragment of a mouse gene is cloned into the Eco RI site of a vector of 6 Kb size, the total length of DNA becomes 14 Kb.
- When digested with EcoRI only two fragments of 8 Kb and 6 Kb are produced, hence two bands are obtained.
- When digested with BamHI only three fragments of 5.5 Kb, 4.5 Kb and 4 Kb are produced, hence three bands are obtained.
- When digested with both EcoRI and BamHI five fragments of 4 Kb, 3.5 Kb, 3 Kb, 2.5 Kb and 1 Kb are produced, hence five bands are obtained.
- The 1 kb, 3 kb and 4 Kb add up to make 8 Kb mouse gene while 3.5 Kb and 2.5 Kb add up to make 6 Kb vector DNA. Hence, 1 kb and 3 kb are fragments of mouse gene.
Regard