The restriction endonuclease Eco R-I recognises and cleaves DNA sequence as shown below
5' - G A A T T C - 3'
3' - C T T A A G - 5'
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence?
A. 10 B. 2 C. 100 D. 50
3' - C T T A A G - 5'
Dear Student.
Please find below the solution to the asked query.
Eco RI is a restriction endonucleases after that cut after 6 bp. i.e (1/4)6 = 1/4096.
This means it cleaves once in 4096 bp.
Its given in question DNA sequence is 10Kb i.e 10000bp
Therefore, 10000/4096 = 2.4.
The correct answer is b. 2
Hope this information will clear your doubts about the topic.
If you have any more doubts just ask here on the forum and our experts will try to help you out as soon as possible.
Regards.
Please find below the solution to the asked query.
Eco RI is a restriction endonucleases after that cut after 6 bp. i.e (1/4)6 = 1/4096.
This means it cleaves once in 4096 bp.
Its given in question DNA sequence is 10Kb i.e 10000bp
Therefore, 10000/4096 = 2.4.
The correct answer is b. 2
Hope this information will clear your doubts about the topic.
If you have any more doubts just ask here on the forum and our experts will try to help you out as soon as possible.
Regards.