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what is a heterologous host??
what is the difference between recombinant and non - recombinant ?
in the year 1963, two enzymes responsible for restricting the growth of E.coli was isolated. how did the enzyme act to restrict the growth of the bacteriophage?
What is the function of renette cells?
Define DNA spooling...?
why does dna cannot pass through the cell membrane?
what is full form of PBR322 vector
When does DNA replication occur in prokaryotic cells?
What are molecular scissors? Explain their role in the process of recombinant DNA technology?
what is the difference between a transformant and recombinant ?
who is father of biotechnology
what is the advantage of a sparged bioreactor over simple stirred bioreactor?
difference between transformant and non transformant
What is "rop" in the cloning vector pBR322.....??
Since DNA is a hydrophilic molecule, it cannot pass through cell membranes.Why
Is enzymes or DNA bigger in molecular size?how do u know?
A linear DNA fragment and a plasmid has three restriction sites for EcoRI
explain the structure of pBR322
What is BamH1??? For what is it used?
in plants, how is alien DNA introduced into the host cell?
meaning of biotechnology acc. to EFB?
Difference between transformant and recombinant?
Traditional hybridization procedures used in plant and animal breeding very often leads toinclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
why are ampR and tetR called as selectable markers ?
what are the functions of selectable markers?
explain insertional inactivation used in the selection of recombinants in biotechnology experimentss ?
what is the difference between endonucleases and exonucleases?
Name the enzyme responsible for the removal of 5-phosphate group from nucleic acid
Provide one sentence information about plasmid with respect to its
What is a reporter enzyme and how can it be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Which one of an intron and an exon is the reminiscent of antiquity???
significance of genetic engineering
At which stage of meiosis a recombinant DNA is made?
significance of genetic engineering
"THE LIGATION OF ALIEN DNA IS CARRIED OUT AT A RESTRICTION SITE PRESENT IN ONE OF THE TWO ANTIBIOTIC RESISTANCE GENE"
What is genetic engineering?Explain Briefly the steps involved in this process?
DIFFERENCE BW ORI AND CLONING SITE IN CLONING VECTORS
explain the features required to facilitate cloning into a vector.......what do you mean by cloning sites......explain briefly...
What's the difference between -galactosidase and Beta-galactosidase?
(b) Restriction enzymes and DNA
name the specific components and the linkage btw them that form deoxyadenosine.
explain the process of elution?
what are strain
Why are engineered vectors preferred by biotechnologists for transferring desired genes into another organism?
What does Pvu I, Pst I, EcoRI,Cla I, Hind III,BamHI, Sal I,Pvu II and rop in the circular figure of cloning vector indicate? How were these names derived?Should we by-heart all these names? What are their functions? How do they get their locations in the diagram?
what is the host that produces a foreign gene product called and what is the foriegn gene product called?
Full form of BAC, YAC and UTR
Give example of molecular scissors?
explain gel electrophoresis
eukaryotic cells have restriction endonucleases? Justify your answer.
notes on biotechnology
What is the difference between recombinant and transformant?
be the molar concentration of human DNA in a human cell? Consult your
which is the best book for hsc 12th ciass of biology
what does non recombinant and non transformant mean?
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